Control group #1
Human peripheral blood lymphocytes were isolated from a healthy 60 years old male donor by differential centrifugation on Histopaque (Sigma-Aldrich Chemicals ltd.) from 30 ml of whole blood obtained via venipuncture of v.cubitale into vacutainers containing anticoagulant (K2), and maintained in RPMI 1640 nutrient with antibiotics and antimycotics.. Four standardized samples of the culture were placed in 2ml phials sealed with a plastic cap and pre-sterilised at 121degrees C under pressure. There was about 0.5 of culture in each sample. All glassware and instruments were handled in a purified air laminar flow unit to avoid bacterial contamination.
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The control group #1 with these vials should simulate a "neutral" location and describes the actual initial sample.
The analysis of ten series of tests showed am average number of:
> 89 viable lymphocytes (green columns)
> 12non-viable lymphocytes (red columns)
>• Study August 2001
>• Control group #1
>• Control group #2
>• Control group #3
>• Control group #4
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